There are many reasons a reaction might fail from the amount of DNA or primer present in the reaction, salt contamination, tech error, or sequencer error. A QC of score of 0 is significant.
There are many reasons a reaction might fail from the amount of DNA or primer present in the reaction, salt contamination, tech error, or sequencer error for instance. Therefore, if your entire order failed and every reaction has a QC of score of 0 this is significant. We will immediately want to review how the order was processed to determine the cause of the failure.
Was big dye added to the reaction? We can look at the sequencing array to help us determine if this is the cause of the failure.
Figure 1 The sequencing array is completely blank. There are no dyeblobs present which indicate to us that Big Dye was missing from the reaction. Therefore, we can attribute the failure to technical error.
Figure 2 Looking at the array for the repeat run, we can clearly see the presence of dyeblobs as Big Dye was added to each well of the plate. This tells us the entire order failed for some other reason unrelated to the presence of big dye. The successful sequence data for the Standard (control) tells us that there is no sequencer or technical error.